■ 基本信息
別名: | pET51b |
啟動子: | T7 |
復制子: | pBR322 |
終止子: | T7 terminator |
質粒分類: | 大腸桿菌載體；PET系列表達質粒 |
質粒大小: | 5218bp |
質粒標簽: | N-Strep-Tag II，N-EK，C-10*His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37℃，有氧，LB |
表達宿主: | BL21(DE3) |
誘導方式: | IPTG或乳糖及其類似物 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pET-51b是一個大腸桿菌pET系列表達質粒。The pET-51b(+) vector carries an N-terminal Strep?Tag II coding sequence (1) followed by a rec-ognition site for enterokinase, and an optional C-terminal His?Tag coding sequence. The presence of two "gentle elution" tags at both the N-terminus and C-terminus is ideal for dual puri?cation strate-gies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below the circle map. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK190pET-51b大腸表達質粒.txt
